Journal of Computing & Biomedical Informatics

Computational Assessment of nsSNPs Associated with FGFR2 Gene

Hira Saleem — 2026-04-18

The fibroblast growth factor receptor 2 (FGFR2) controls cell proliferation, differentiation, angiogenesis, and wound healing. Single nucleotide polymorphisms (SNPs) of the FGFR2 gene are linked with Pfeiffer syndrome, Crouzon syndrome, and Jackson-Weiss syndrome. Missense mutations in FGFR2 have also been reported in breast, gastric, and lung cancer. Objective: This study aimed to systematically analyze missense SNPs (nsSNPs) in FGFR2 using an integrative computational pipeline to identify variants with the strongest predicted pathogenic impact. Methods: FGFR2 gene data and protein sequence were retrieved from the NCBI dbSNP [build 153 (March 2020)] and UniProt database [release 2020_02; UniProt ID: P21802]. A total of eleven bioinformatics tools—SIFT, PolyPhen-2, PROVEAN, SNAP2, SNPs&GO, PANTHER, PhD-SNP, PMut, I-Mutant3.0, ConSurf server, and Project HOPE—were used to examine the deleterious potential of nsSNPs. The interaction of FGFR2 with different genes was analyzed using GeneMANIA. Results: We investigated 28,027 total SNPs from dbSNP, of which 701 were coding variants: 294 synonymous and 407 non-synonymous. Among the latter, 393 were missense mutations, 7 frameshift mutations, and 7 nonsense mutations. Only missense nsSNPs were retained for further analysis. Stepwise filtering identified 90 consensus-deleterious variants (≥ 6/8 predictors), 54 extremely damaging variants (all 8 predictors), and 38 unstable variants (ΔΔG ≤ −0.5 kcal/mol). A final set of 24 highly conserved and damaging variants (ConSurf score ≥7) was prioritized. Conclusion: A total of 24 nsSNPs (L757S, G690R, P666S, R664W, D644N, Y616C, L572F, L550P, L550V, I547M, V514M, G502E, G502R, E489K, R450C, P286S, C278Y, G271R, P263L, P256S, D225E, S224P, E219G, and Y105C) were predicted to have the most damaging and disease-causing effects on FGFR2 protein function and structure. Thus, the early prediction of FGFR2 gene functions could aid in disease prognosis. The results of our study provide beneficial information for devising early diagnostic and therapeutic measures.

Analyzing the Impact of Pretrained Language Models on Low-Resource Languages

Muhammad Irshad Hussain — 2026-04-18

The rapid advancement of natural language processing has predominantly benefited high-resource languages such as English, Chinese, and Spanish, leaving thousands of languages underserved. This digital language divide limits equitable access to technology and threatens global linguistic diversity. This paper presents a systematic evaluation of eight pretrained language models across seven low-resource languages representing five distinct language families. Through extensive experiments on sentiment analysis, named entity recognition, and machine translation tasks, we demonstrate that multilingual BERT achieves the highest average accuracy of 74.5%. We further propose a novel adaptation framework combining vocabulary augmentation, continual pretraining, task-adaptive fine-tuning, and knowledge distillation that improves performance by up to 18.7%. Our analysis identifies vocabulary overlap as the strongest predictor of cross-lingual transfer success, explaining 76.3% of performance variance. These findings provide evidence-based guidelines for researchers and practitioners developing inclusive NLP technologies for underserved language communities. Limitations of this study include the focus on seven languages (generalizability to other low-resource languages requires further validation), computational constraints that prevented evaluation of models exceeding 300M parameters, and potential biases introduced by dataset availability and quality across languages.

Multi-Class Classification of Alzheimers Impairment and Dementia Stages using an Efficientnet-B0 Deep Learning Framework

Makki Riaz Khan — 2026-04-18

Alzheimer’s disease is a progressive neurodegenerative condition that leads to deterioration of memory and cognitive function, where early detection is critical as therapeutic interventions are most effective prior to extensive neuronal loss. Many existing deep learning approaches focus on binary or ternary classification schemes, which inadequately reflect the full clinical spectrum of cognitive impairment and dementia, thereby limiting their practical diagnostic relevance.This study proposes a lightweight deep learning framework based on EfficientNet-B0 for fine-grained eight-class Alzheimer’s staging, encompassing four levels of cognitive impairment and four levels of dementia. A structured preprocessing pipeline, including normalization, contrast enhancement, resizing, and targeted data augmentation, is employed to improve MRI consistency and enhance discriminative feature learning.Under a strict patient-level evaluation protocol, the proposed model achieves a peak testing accuracy of 99.78% and demonstrates strong and stable performance when compared with commonly used 2D convolutional neural network baselines. Owing to its low computational complexity and consistent performance across classes, the framework represents a promising research-stage tool for automated Alzheimer’s disease staging and warrants further validation, including comparison with transformer-based and volumetric approaches, before clinical deployment.

Intelligent Healthcare Chatbot to Enhance Patient Satisfaction and Engagement with Implementation of Advance HCI Techniques

Muhammad Khalid — 2026-04-18

Effective communication is crucial to the quality of healthcare delivery, whereas communication breakdowns between patients and healthcare providers are an ongoing issue. AI Chatbot have proven to be a potential solution to enhance patient interaction and satisfaction through availability and customization. The purpose of this research was to develop and evaluate an AI-based healthcare Chatbot, created in accordance with the principles of advanced HCI, to improve patient interdependence and satisfaction and to compare the performance of the developed Chatbot with the existing healthcare Chatbot. The Chatbot has been created based on publicly available healthcare datasets in Kaggle, and preprocessing, including cleaning and parting of the data and correction of spelling mistakes, have been done. Principles of HCI like usability, accessibility, error handling, and user feedback were taken into consideration and performance determined by usability testing of 150 users, engagement metrics evaluation, and deep-learning testing using accuracy, precision, and recall. It was found that usability was very high (88% of tasks in 12.5 seconds on average, 8% error rate), user satisfaction was 4.6/5 on average, and 85% were of the opinion that the interface was easy to use. The engagement metrics showed that the average session time was 4.5 minutes, 78% of tasks completed and 62% retention rate. The deep learning model was 91.3 %, 89.7 %, and 87.5 % accurate, precise, and recalled, respectively, in terms of the ability to interpret patient requests. Altogether, the HCI-based AI Chatbot enhanced patient engagement and satisfaction considerably through usability, accessibility, and receptive interactions, the error management, individualized communication, and multimodal interface, which supported effective patient-providing communication. The next idea that should be incorporated in the future work is to combine multilingual support, voice-based interaction, and connectivity with the electronic health record in order to increase its efficacy.

Cybersecurity Architecture for Medical Live Monitoring Systems Using HTTP Protocols and SHA-256 Encryption

J. Dafni Rose — 2026-03-01

Live health monitoring systems are revolutionizing patient care, yet the security and integrity of sensitive medical data remain a critical challenge. To address the vulnerabilities of centralized databases and basic authentication in traditional systems, we propose a secure framework that leverages a decentralized architecture. This proposed system uses standard HTTPS protocols for communication, with each client request authenticated using JSON Web Tokens (JWT) signed with HMAC-SHA256. Patient records are encrypted using AES-256-GCM before storage, with cryptographic hashes of these records written to a private Hyperledger Fabric blockchain for tamper-proof auditability. A smart contract enforces decentralized, role-based access control to ensure only authorized personnel can view sensitive information. A predictive analytics module processes secured vital data to forecast potential health deterioration, triggering automated alerts to the responsible physician. In a simulation handling 1,000 patient records over 30 days, the system demonstrated 99.98% data integrity, processed an average of 4,820 authenticated requests per minute with a mean latency of 185ms, and achieved 87.3% accuracy in predicting critical health events with a 30-minute lead time, confirming its efficacy as a robust and secure solution for remote patient monitoring.

Quantum-Safe Wireless Sensor Networks: A Post-Quantum Cryptography Framework with Adaptive Security Optimization

Siri D — 2026-03-01

Quantum computing poses a significant threat to Wireless Sensor Networks (WSNs) by undermining traditional cryptographic algorithms such as RSA and Elliptic Curve Cryptography (ECC). This work proposes a quantum-safe architecture for WSNs that integrates post-quantum cryptography (PQC) with lightweight IoT protocols to ensure long-term confidentiality, authenticity, and resilience. The framework leverages ML-KEM for key encapsulation and ML-DSA/SLH-DSA for signatures, and seamlessly integrates with EDHOC, OSCORE, and COSE. A novel Efficient Adaptive Parameter Selection (EAPS) mechanism dynamically adjusts cryptographic strength to balance security, energy consumption, and latency under varying network conditions. Experimental evaluation demonstrates that ciphertext fragmentation (8–27 pieces) results in manageable completion times of 4–10 seconds, while join operations consume only 0.005–0.03 J per cryptographic handshake event, while system-level energy consumption including network overhead is higher (~1.0–1.4 J per join cycle depending on hop count and retransmissions). Battery lifetime projections under steady-state sensing workloads range from approximately 115–280 months for low-duty-cycle operation, whereas realistic deployment conditions with periodic communication yield effective lifetimes of 11.5–24 months. Security analysis confirms robust resistance against downgrade attacks and Harvest-Now-Decrypt-Later (HNDL) threats. Moreover, EAPS reduces risk scores by over 50% compared to fixed schemes with less than 10% additional energy overhead, and batch signature verification improves scalability by increasing throughput from ~1,600 to ~2,800 verifications per second. Overall, the proposed framework demonstrates that WSNs can achieve quantum-resistant security with minimal performance trade-offs, ensuring readiness for the post-quantum era.

A Hybrid Trust Evaluation Framework for Cloud Service Providers in Cybersecurity-Critical Environments

Mamoon Obiedat — 2026-03-01

The rapid expansion of cloud computing has transformed digital service delivery, but establishing the trustworthiness of Cloud Service Providers (CSPs) remains a significant challenge, especially for sensitive workloads. This study presents a novel hybrid evaluation framework aimed at quantifying CSP trustworthiness from multiple perspectives. The framework combines a deterministic Multi-Attribute Trust Model (MATM) with a sophisticated Fuzzy Inference System (FIS). The MATM assesses CSPs based on three key attributes: Cost Efficiency (CE), Performance (P), and Reputation & Trustworthiness (RT). It utilizes a weighted normalization function to compute a transparent Trustworthiness Level (TL), offering a clear quantitative benchmark. To handle the inherent uncertainty and subjectivity in trust evaluation, the framework incorporates an FIS that uses fuzzy logic to interpret linguistic variables and capture the complex relationships among attributes. This dual approach provides both a straightforward, scalable assessment method and a more nuanced, human-centric evaluation. The framework’s effectiveness was validated through scenario analysis. As an initial proof of concept, the evaluation utilized constructed provider scenarios representing typical market profiles. The results demonstrate the model’s ability to clearly differentiate between service offerings, confirming its utility as a comprehensive decision-support tool.